Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells.
Identifieur interne : 005281 ( Main/Exploration ); précédent : 005280; suivant : 005282Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells.
Auteurs : Zhi Wang [République populaire de Chine] ; Lili Ren ; Xingang Zhao ; Tao Hung ; Anming Meng ; Jianwei Wang ; Ye-Guang ChenSource :
- Journal of virology [ 0022-538X ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : ARN viral.
- génétique : Virus du SRAS.
- pharmacologie : Petit ARN interférent.
- physiologie : Virus du SRAS.
- Animaux, Cellules Vero, Effet cytopathogène viral, Humains, Interférence par ARN, Réplication virale, Virus du SRAS.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : RNA, Viral.
- chemical , pharmacology : RNA, Small Interfering.
- drug effects : Cytopathogenic Effect, Viral, SARS Virus, Virus Replication.
- genetics : SARS Virus.
- physiology : SARS Virus.
- Animals, Chlorocebus aethiops, Humans, RNA Interference, Vero Cells.
Abstract
Severe acute respiratory syndrome (SARS) is an acute respiratory infectious disease that spread worldwide in early 2003. The cause was determined as a novel coronavirus (CoV), SARS-associated CoV (SARS-CoV), with a single-stranded, plus-sense RNA. To date, no effective specific treatment has been identified. To exploit the possibility of using RNA interference as a therapeutic approach to fight the disease, plasmid-mediated small interfering RNAs (siRNAs) were generated to target the SARS-CoV genome. The expression of siRNAs from two plasmids, which specifically target the viral RNA polymerase, effectively blocked the cytopathic effects of SARS-CoV on Vero cells. These two plasmids also inhibited viral replication as shown by titer assays and by an examination of viral RNA and protein levels. Thus, our results demonstrated the feasibility of developing siRNAs as effective anti-SARS drugs.
DOI: 10.1128/JVI.78.14.7523-7527.2004
PubMed: 15220426
Affiliations:
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Le document en format XML
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<term>Chlorocebus aethiops</term>
<term>Cytopathogenic Effect, Viral (drug effects)</term>
<term>Humans</term>
<term>RNA Interference</term>
<term>RNA, Small Interfering (pharmacology)</term>
<term>RNA, Viral (biosynthesis)</term>
<term>SARS Virus (drug effects)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (physiology)</term>
<term>Vero Cells</term>
<term>Virus Replication (drug effects)</term>
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<term>Effet cytopathogène viral ()</term>
<term>Humains</term>
<term>Interférence par ARN</term>
<term>Petit ARN interférent (pharmacologie)</term>
<term>Réplication virale ()</term>
<term>Virus du SRAS ()</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (physiologie)</term>
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<term>Chlorocebus aethiops</term>
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<term>RNA Interference</term>
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<term>Effet cytopathogène viral</term>
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is an acute respiratory infectious disease that spread worldwide in early 2003. The cause was determined as a novel coronavirus (CoV), SARS-associated CoV (SARS-CoV), with a single-stranded, plus-sense RNA. To date, no effective specific treatment has been identified. To exploit the possibility of using RNA interference as a therapeutic approach to fight the disease, plasmid-mediated small interfering RNAs (siRNAs) were generated to target the SARS-CoV genome. The expression of siRNAs from two plasmids, which specifically target the viral RNA polymerase, effectively blocked the cytopathic effects of SARS-CoV on Vero cells. These two plasmids also inhibited viral replication as shown by titer assays and by an examination of viral RNA and protein levels. Thus, our results demonstrated the feasibility of developing siRNAs as effective anti-SARS drugs.</div>
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<name sortKey="Meng, Anming" sort="Meng, Anming" uniqKey="Meng A" first="Anming" last="Meng">Anming Meng</name>
<name sortKey="Ren, Lili" sort="Ren, Lili" uniqKey="Ren L" first="Lili" last="Ren">Lili Ren</name>
<name sortKey="Wang, Jianwei" sort="Wang, Jianwei" uniqKey="Wang J" first="Jianwei" last="Wang">Jianwei Wang</name>
<name sortKey="Zhao, Xingang" sort="Zhao, Xingang" uniqKey="Zhao X" first="Xingang" last="Zhao">Xingang Zhao</name>
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